Prediction of Radioresistant Prostate Most cancers Based mostly on Differentially Expressed Proteins

Introduction: 

Though relapses after radiotherapy are widespread in prostate most cancers (PCA) sufferers, these with a excessive threat for radioresistance can’t be recognized previous to therapy but. Subsequently, this proof-of-concept research was carried out to check protein expression profiles of sufferers with radio-recurrent PCA to sufferers handled with major radical prostatectomy separated by Gleason threat teams. We hypothesized that radio-recurrent PCA have an analogous protein expression as high-risk Gleason PCA.

Strategies:

Affected person cohorts consisted of (i) 31 sufferers handled with salvage prostatectomy for regionally recurrent PCA after major radiotherapy and (ii) 94 sufferers handled with major prostatectomy break up right into a Gleason high-risk (≥4 + 3; n = 42 [44.7%]) versus a low-risk group (≤3 + 4; n = 52 [55.3%]). Immunohistochemistry was carried out utilizing 15 antibodies with identified affiliation to radioresistance in PCA in vitro. ELISA was used for validation of chosen markers in serum.

Outcomes: 

Androgen receptor (AR) was overexpressed in most radio-recurrent PCA (89.7%) and in most major high-risk Gleason PCA (87.8%; p = 0.851), whereas solely 67.3% of the low-risk group confirmed an expression (p = 0.017). Contemplating the best Gleason sample in major PCA, aldo-keto reductase household 1 member C3 (AKR1C3) was most equally expressed by sufferers with radio-recurrent PCA and sufferers with Gleason patterns Four and 5 (p = 0.827 and p = 0.893) in comparison with Gleason sample 3 (p = 0.20). These findings had been supported by ELISA.

Conclusion:

That is the primary research to judge protein markers with a purpose to predict radioresistance in PCA. Our outcomes level to AR and AKR1C3 as essentially the most promising markers which may assist stratify sufferers for radiotherapy.

Human CCL-14
hAP-0138A	Angio Proteomie	100ug	EUR 250

Anti-Human CCL-11 Antibody
101-M161	ReliaTech	100 µg	EUR 399
Description: CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the aminoterminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP1, 2, and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.

Anti-Human CCL-14 Antibody
101-M162	ReliaTech	100 µg	EUR 399
Description: CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20-93) is a 74 amino acid peptide that is also known as HCC1 (Hemofiltrate CC Chemokine1). It is a weak CCR1 agonist, however, an 8 amino acid Nterminal truncation (aa 28 93) allows potent signaling through CCR1 and CCR5. CCL14b, also called HCC3, is a 90 amino acid peptide (aa 20-109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Anti-Human CCL-28 Antibody
101-M185	ReliaTech	100 µg	EUR 399
Description: CCL-28 or MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.

Anti-Human CCL-14 Antibody
101-M246	ReliaTech	100 µg	EUR 399
Description: CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20-93) is a 74 amino acid peptide that is also known as HCC1 (Hemofiltrate CC Chemokine1). It is a weak CCR1 agonist, however, an 8 amino acid Nterminal truncation (aa 28 93) allows potent signaling through CCR1 and CCR5. CCL14b, also called HCC3, is a 90 amino acid peptide (aa 20-109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Anti-Human CCL-11 Antibody
101-M256	ReliaTech	100 µg	EUR 399
Description: CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the aminoterminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP1, 2, and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.

Anti-Human CCL-13 Antibody
101-M257	ReliaTech	100 µg	EUR 399
Description: CCL13, also known as Monocyte Chemoattractant Protein4 (MCP4), is a CC chemokine that acts as a chemoattractant for monocytes, eosinophils and T cells and as an activator of basophils. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. The bioactivities of CCL13 are mediated by the CC chemokine receptors CCR2 and CCR3.

Anti-Human CCL-16 Antibody
101-M259	ReliaTech	100 µg	EUR 399
Description: CCL16, also known as HCC-4, is a CC chemokine that interacts with CCR1, CCR2, and CCR5. CCL16 is expressed weakly by NK cells, γδ T cells, and some T cells and is upregulated in monocytes by IL-10. CCL16 functions as a chemoattractant for monocytes and promotes the maturation of monocyte derived dendritic cells.

Anti-Human CCL-17 Antibody
101-M260	ReliaTech	100 µg	EUR 399
Description: CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP1α, MIP1β, MCP1, MCP2, MCP3, and I309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

Anti-Human CCL-18 Antibody
101-M261	ReliaTech	100 µg	EUR 399
Description: CCL18, also known as PARC (Pulmonary and Activation-regulated Chemokine), is expressed by monocyte-derived dendritic cells and Th2 cytokine-stimulated macrophages. It functions as a chemoattractant for naïve T cells but not monocytes or neutrophils.

Anti-Human CCL-19 Antibody
101-M262	ReliaTech	100 µg	EUR 399
Description: CCL19, also known as MIP3β and ELC (EBI1Ligand Chemokine), is a 77 amino acid (aa) β chemokine that is distantly related to other β chemokines (20-30% aa sequence identity). The gene for MIP3β has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human β chemokines are clustered. MIP3β is constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP3β is downregulated by the antiinflammatory cytokine IL10. Recombinant MIP3β is chemotactic for cultured human lymphocytes. MIP3β is a ligand for CCR7 (previously referred to as the EpsteinBarr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is strongly upregulated in B cells infected with EpsteinBarr virus and T cells infected with herpesvirus 6 or 7.

Anti-Human CCL-20 Antibody
101-M264	ReliaTech	100 µg	EUR 399
Description: CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel β chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other β chemokines (20-28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR6 (previously referred to as GPRCY4, CKRL3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

Anti-Human CCL-21 Antibody
101-M265	ReliaTech	100 µg	EUR 399
Description: 6Ckine or CCL-21 is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (Secondary Lymphoid-tissue Chemokine), CCL21 and Exodus2, shows 21 33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of β chemokines plus two additional cysteines in its unusually long carboxy-lterminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the gene for MIP3β/ ELC, another novel CC chemokine.

Anti-Human CCL-22 Antibody
101-M266	ReliaTech	100 µg	EUR 399
Description: CCL22, also named stimulated T cell chemotactic protein (STCP1) and MDC, is a CC chemokine initially isolated from clones of monocytederived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in dendritic cells, IL2 activated NK cells, and activated T lymphocytes. A CD8+ T lymphocytederived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV1 isolates and the T cell lineadapted isolate HIV1 IIIB, has been identified as CCL22. Based on aminoterminal sequence analysis, the major CD8 + T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.

Anti-Human CCL-23 Antibody
101-M267	ReliaTech	100 µg	EUR 399
Description: CCL23, also known as Myeloid Progenitor Inhibitory Factor1 (MPIF1), is a CC chemokine that is produced by some myeloid cell lines and monocytederived dendritic cells. It binds CCR1 and inhibits the differentiation of progenitor cells that give rise to granulocytes and macrophages. CCL23 is also chemotactic for resting T cells, neutrophils, monocytes and dendritic cells.

Anti-Human CCL-24 Antibody
101-M268	ReliaTech	100 µg	EUR 399
Description: Eotaxin2, also named MPIF2 and Ckβ6, is a CC chemokine that is designated CCL24. Eotaxin2 cDNA encodes a 10.5 kDa, 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. C-terminally truncated variants with 78, 73, 75 and 76 residues have also been described. Eotaxin 2 shares 40%, 42% and 39% amino acid sequence identity with other CC chemokines CCL7/MCP3, CCL3/MIP1α, and CCL11/Eotaxin, respectively. Eotaxin2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes, but not monocytes or activated T lymphocytes. Eotaxin2 also suppresses colony formation by high proliferative multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin2, Eotaxin and CCL13/MCP4 are mediated by the receptor CCR3.

Anti-Human CCL-25 Antibody
101-M269	ReliaTech	100 µg	EUR 399
Description: CCL25 (thymus-expressed chemokine) is a CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 aa precursor protein with a 23 aa signal peptide that is cleaved to yield a 127 aa mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 aa protein that exhibits 49% aa sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 are chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 is also a specific agonist for the CCR9 chemokine receptor.

Anti-Human CCL-26 Antibody
101-M270	ReliaTech	100 µg	EUR 399
Description: Eotaxin-3 is a CC chemokine that signals through the CCR3 receptor. It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells and monocytes. Eotaxin-3 has similar activity to Eotaxin and Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Recombinant human Eotaxin-3 is an 8.4 kDa protein containing 71 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Anti-Human CCL-27 Antibody
101-M271	ReliaTech	100 µg	EUR 399
Description: CTACK or CCL-27 is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues. Recombinant human CTACK is a 10.2 kDa protein containing 88 amino acid residues.

Anti-Human CCL-28 Antibody
101-M272	ReliaTech	100 µg	EUR 399
Description: CCL-28 or MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.

Anti-Human CCL-1 Antibody
101-M255	ReliaTech	100 µg	EUR 399
Description: ccbe1 is not expressed in endothelial cells of lymph vessels, and it may be a component of the extracellular matrix. In zebrafish, ccbe1 expression was observed along the earliest migra­tion routes of endothelial cells that sprout from the posterior cardinal vein and migrate circuitously before developing into lymphatic vessels. ccbe1 might therefore be involved in guidance of these migrating cells.

Anti-Human CCL-2 Antibody
101-M263	ReliaTech	100 µg	EUR 399
Description: CCL2 is a βchemokine that is also known as monocyte chemotactic protein 1 (MCP1). It is produced by a variety of cell types and is chemotactic for monocytes.

Anti-Human CCL-3 Antibody
101-M273	ReliaTech	100 µg	EUR 399
Description: The macrophage inflammatory proteins 1α and 1β were originally copurified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1α refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP 1α. Mature MIP1α and MIP1β in both human and mouse share approximately 70% homology at the amino acid level. The MIP1 proteins are members of the β (CC) subfamily of chemokines. Both MIP1α and MIP1β are monocyte chemoattractants in vitro. Additionally, the MIP1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP1α and MIP1β preferentially attracting CD8+ and CD4+ T cells, respectively. MIP1α has also been shown to attract B cells as well as eosinophils. MIP1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP1α has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP1α has been identified as CCR1 and CCR5.

Anti-Human CCL-5 Antibody
101-M274	ReliaTech	100 µg	EUR 399
Description: CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the β (CC) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3 and 5.

Anti-Human CCL-7 Antibody
101-M275	ReliaTech	100 µg	EUR 399
Description: MCP2 and CCL7 are two monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and CCL7 are members of the CC family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. CCL7 also shares 58% amino acid identity with MCP2. CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites. Similarly to other CC chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Anti-Human CCL-8 Antibody
101-M276	ReliaTech	100 µg	EUR 399
Description: CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.

Anti-Human CCL-17 (TARC) Antibody
101-M164	ReliaTech	100 µg	EUR 399
Description: CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP1α, MIP1β, MCP1, MCP2, MCP3, and I309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

Anti-Human CCL-27 (CTACK) Antibody
101-M184	ReliaTech	100 µg	EUR 399
Description: CTACK or CCL-27 is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues. Recombinant human CTACK is a 10.2 kDa protein containing 88 amino acid residues.

Mouse Monoclonal anti-Human CCL-11
hAP-0136	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-Human CCL-11
hAP-0136A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-28
hAP-0153	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-28
hAP-0153A	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-28
hAP-5717	Angio Proteomie	50ug	EUR 400

Anti-Human CCL-5 (RANTES) Antibody
101-M191	ReliaTech	100 µg	EUR 399
Description: CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the β (CC) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3 and 5.

Human RANTES (CCL 5)
MBS692044-0005mg	MyBiosource	0.005mg	EUR 300

Human RANTES (CCL 5)
MBS692044-002mg	MyBiosource	0.02mg	EUR 450

Human RANTES (CCL 5)
MBS692044-5x002mg	MyBiosource	5x0.02mg	EUR 1725

Mouse Monoclonal anti-Human CCL-1
hAP-0135	Angio Proteomie	100ug	EUR 250

Human CCL-5 ELISA Kit
EHC0240	Abclonal	96Tests	EUR 625.2

Anti-Mouse CCL-11 Antibody
103-M321	ReliaTech	100 µg	EUR 399
Description: CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Mouse CCL11 cDNA encodes a 97 amino acid (aa) precursor protein from which the aminoterminal 23 aa are cleaved to generate the 74 aa mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high aa sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFNγ. In addition, CCL11 mRNA is also induced in response to the transplantation of IL4secreting tumor cells. The CC chemokine receptor 3 (CCR3) has been identified as a specific human CCL11 receptor.

Anti-Mouse CCL-12 Antibody
103-M322	ReliaTech	100 µg	EUR 399
Description: CCL12 is a cloned mouse CC chemokine most closely related to human MCP1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

Anti-Mouse CCL-17 Antibody
103-M323	ReliaTech	100 µg	EUR 399
Description: Human thymus and activationregulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse CCL17 was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse CCL17 cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP1α, MIP1β, MCP1, MCP2, MCP3, and I309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse CCL17 is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant CCL17 has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, CCL17 was identified to be a specific functional ligand for CCR4 and CCR8, receptors that are selectively expressed on T cells.

Anti-Mouse CCL-19 Antibody
103-M324	ReliaTech	100 µg	EUR 399
Description: CCL19/MIP3β, also known as ELC (EBI1Ligand Chemokine), is a β chemokine that binds specifically to the chemokine receptor CCR7/EBI1/ BLR2. Mouse (human) CCL19 cDNA encodes a 108 (98) amino acid precursor protein with a predicted 25 (21) aa signal peptide that is cleaved to form the 83 (77) aa mature secreted protein. CCL19 is distantly related to other β chemokines (20-30% aa sequence identity). Mouse CCL19 shares 83% aa sequence homology with human CCL19. CCL19 has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of CCL19 is downregulated by the antiinflammatory cytokine IL10. Recombinant CCL19 has been shown to be chemotactic for Tcells and Bcells. The CCL19 receptor (CCR7/ EBI1/ BLR2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly upregulated in Bcells infected with EpsteinBarr virus and T-cells infected with herpesvirus 6 or 7.

Anti-Mouse CCL-20 Antibody
103-M326	ReliaTech	100 µg	EUR 399
Description: Macrophage inflammatory protein 3 alpha (MIP-3α) is a member of the CC chemokine superfamily and has been designated CCL20. CCL20 is a β chemokine that is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. CCL20 binds to CCR6 to promote chemotaxis of lymphocytes and inhibit the proliferation of myeloid progenitors.

Anti-Mouse CCL-21 Antibody
103-M327	ReliaTech	100 µg	EUR 399
Description: 6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoidtissue chemokine), CCL21 and Exodus2, shows 21-33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of β chemokines plus two additional cysteines in its unusually long carboxy-lterminal domain. Human 6Ckine cDNA encodes a 134 amino acid highly basic precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine share 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6Ckine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dosedependent manner. 6Ckine acts via the CC receptor CCR7 on T cells and B cells.

Anti-Mouse CCL-22 Antibody
103-M328	ReliaTech	100 µg	EUR 399
Description: CCL22, also known as ABCD1 and MDC (macrophage-derived chemokine), is a CC chemokine cloned from activated mouse B cells. Mouse CCL22 cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human CCL22 share 64% identity and 83% similarity. The genomic organization of the mouse and human CCL22 genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse CCL22 is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse CCL22 mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. CCL22 is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

Anti-Mouse CCL-24 Antibody
103-M329	ReliaTech	100 µg	EUR 399
Description: Eotaxin2, also named myeloid progenitor inhibitory factor (MPIF2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL4. LPS and IL4 also differentially regulate the expression of Eotaxin2 in monocytes and macrophages. Mouse Eotaxin2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin2. Functionally, Eotaxin2is most closely related to CCL11/Eotaxin and CCL26/Eotaxin3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin.

Anti-Mouse CCL-25 Antibody
103-M330	ReliaTech	100 µg	EUR 399
Description: CCL25 (thymus expressed chemokine), also known as TECK (thymus-expressed chemokine), is a CC chemokine that is distantly related (twenty some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein. Mouse CCL25 shares 49% amino acid sequence identity with human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8.

Anti-Mouse CCL-27 Antibody
103-M331	ReliaTech	100 µg	EUR 399
Description: CCL27, also known as CTACK (cutaneous T cel-lattracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines. Mature mouse CCL27 is a 95 amino acid (aa) protein that shares 57% aa and 87% aa sequence identity with human and rat CCL27, respectively. It shares 18-31% aa sequence identity with other mouse CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration. CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin. CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL1, and determinants on the surface of fibroblasts and endothelial cells. CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation. Endothelial cellbound CCL27 can mediate the adhesion of those cells to CLA+ T cells. CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing. In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides.

Anti-Mouse CCL-28 Antibody
103-M332	ReliaTech	100 µg	EUR 399
Description: Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) precursor protein with a 22 aa signal peptide and a 108 aa mature protein. Mature human and mouse CCL28 share 83% aa identity. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10, which is also the receptor for CCL27/CTACK.

Anti-Human CCL-23 (MPIF-1) Antibody
101-M183	ReliaTech	100 µg	EUR 399
Description: CCL23, also known as Myeloid Progenitor Inhibitory Factor1 (MPIF1), is a CC chemokine that is produced by some myeloid cell lines and monocytederived dendritic cells. It binds CCR1 and inhibits the differentiation of progenitor cells that give rise to granulocytes and macrophages. CCL23 is also chemotactic for resting T cells, neutrophils, monocytes and dendritic cells.

Mouse Monoclonal anti-human CCL-22 (MDC)
hAP-0147	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-17 (TARC)
hAP-0141	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-17 (TARC)
hAP-0141A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-18 (PARC)
hAP-0142	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-25 (TECK)
hAP-0150	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-17 (TARC)
hAP-5708	Angio Proteomie	50ug	EUR 400

Sheep Polyclonal anti-Human CCL-18 (PARC)
hAP-5709	Angio Proteomie	50ug	EUR 400

Sheep Polyclonal anti-Human CCL-25 (TECK)
hAP-5714	Angio Proteomie	50ug	EUR 400

Anti-Mouse CCL-1 Antibody
103-M320	ReliaTech	100 µg	EUR 399
Description: Mouse CCL1, also known as TCA3, is a member of the CC beta family of chemokines. The human chemokine I309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA3. Mouse TCA3 and human I309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines. CCL1 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of CCL1 contains one potential Nlinked glycosylation site. Mouse CCL1 is found on the distal portion of mouse chromosome 11 in a cluster with MIP1α, MIP1β and JE. CCL1 acts by binding to the seven transmembrane spanning G-protein coupled receptor, CCR8. CCL1 has been shown to chemoattract T cells.

Anti-Mouse CCL-2 Antibody
103-M325	ReliaTech	100 µg	EUR 399
Description: Mouse CCL2 is a member of the β (CC) subfamily of chemokines. The mouse CCL2 gene was initially identified as a plateletderived growth factorinducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP1. Compared to human MCP1, mouse CCL2 has a 49 aa residue extension at the carboxyterminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C terminal extension could be due either to purification artifact or to posttranslational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP1 has limited activity on mouse cells.

Anti-Mouse CCL-3 Antibody
103-M333	ReliaTech	100 µg	EUR 399
Description: The macrophage inflammatory proteins 1α (or CCL3) and 1β, two closely related but distinct proteins, were originally co purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1α shares approximately 77% and 70% amino acid identity with human MIP1α and mouse MIP1β, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins are members of the β (CC) subfamily of chemokines. Both MIP1α and MIP1β are monocyte chemoattractants in vitro. Additionally, the MIP1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP1α and MIP1β preferentially attracting CD8+ and CD4+ T cells, respectively. MIP1α has also been shown to attract B cells as well as eosinophils. MIP1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP1α has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP1β was reported to be much less active. The functional receptor for MIP1α has been identified as CCR1 and CCR5.

Anti-Mouse CCL-4 Antibody
103-M334	ReliaTech	100 µg	EUR 399
Description: CCL4, also known as macrophage inflammatory protein 1 beta (MIP1β), is a 12 kDa β chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP1α. The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV. Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of Mtropic HIV1 which utilizes CCR5 as a coreceptor. Both forms of CCL4 block HIV1 infection of T cells by inducing the downregulation of CCR5. Mature mouse CCL4 shares 77% and 86% amino acid sequence identity with human and rat CCL4, respectively.

Anti-Mouse CCL-5 Antibody
103-M335	ReliaTech	100 µg	EUR 399
Description: CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa βchemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes. Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% amino acid (aa) sequence identity with rat CCL5 and 75-88% with canine, cotton rat, feline, and human CCL5. CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5. Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28. In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M tropic) but not X4 (T tropic) strains of HIV. The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M tropic HIV 1 infection of monocytes. Oligomerization of CCL5 on glycosaminoglycans is required for CCR1mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4. The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes. CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature.

Anti-Mouse CCL-6 Antibody
103-M336	ReliaTech	100 µg	EUR 399
Description: Mouse CCL6, a member of the β subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11.

Anti-Mouse CCL-8 Antibody
103-M337	ReliaTech	100 µg	EUR 399
Description: CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.

Anti-Mouse CCL-9 Antibody
103-M338	ReliaTech	100 µg	EUR 399
Description: Mouse CCL9/10 (also named MIP1γ and MRP2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the β (or CC) intercrine family of chemokines. Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteinecontaining CC subfamily of chemokines. This subfamily contains four Nterminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no humanto rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded Nterminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines. Mouse CCL9/10 is 75% aa identical to rat CCL9/10. Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at fulllength, but are converted to highly active forms upon Nterminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the Nterminus, generating a highly active, 8 kDa, 71 73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP1α and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a fulllength molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants.

Mouse Monoclonal anti-human CCL-27 (CTACK)
hAP-0152	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-27 (CTACK)
hAP-0152A	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-27 (CTACK)
hAP-5716	Angio Proteomie	50ug	EUR 400

Mouse Monoclonal anti-human CCL-21 (6Ckine)
hAP-0146	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-21 (6Ckine)
hAP-5711	Angio Proteomie	50ug	EUR 400

Mouse Monoclonal anti-human CCL-24 (Eotaxin)
hAP-0149	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-11 (Eotaxin)
hAP-5703	Angio Proteomie	50ug	EUR 400

Sheep Polyclonal anti-Human CCL-24 (Eotaxin)
hAP-5713	Angio Proteomie	50ug	EUR 400

Anti-Human CCL-3 (MIP1 alpha) Antibody
101-M186	ReliaTech	100 µg	EUR 399
Description: The macrophage inflammatory proteins 1α and 1β were originally copurified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1α refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP 1α. Mature MIP1α and MIP1β in both human and mouse share approximately 70% homology at the amino acid level. The MIP1 proteins are members of the β (CC) subfamily of chemokines. Both MIP1α and MIP1β are monocyte chemoattractants in vitro. Additionally, the MIP1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP1α and MIP1β preferentially attracting CD8+ and CD4+ T cells, respectively. MIP1α has also been shown to attract B cells as well as eosinophils. MIP1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP1α has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP1α has been identified as CCR1 and CCR5.

Anti-Human CCL-3 (MIP1 alpha) Antibody
101-M187	ReliaTech	100 µg	EUR 399
Description: The macrophage inflammatory proteins 1α and 1β were originally copurified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1α refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP 1α. Mature MIP1α and MIP1β in both human and mouse share approximately 70% homology at the amino acid level. The MIP1 proteins are members of the β (CC) subfamily of chemokines. Both MIP1α and MIP1β are monocyte chemoattractants in vitro. Additionally, the MIP1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP1α and MIP1β preferentially attracting CD8+ and CD4+ T cells, respectively. MIP1α has also been shown to attract B cells as well as eosinophils. MIP1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP1α has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP1α has been identified as CCR1 and CCR5.

Mouse Monoclonal anti-human CCL-5 (RANTES)
hAP-0155	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-5 (RANTES)
hAP-0155A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-13 (MCP-4)
hAP-0137	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-16 (HCC-4)
hAP-0140	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-13 (MCP-4)
hAP-5704	Angio Proteomie	50ug	EUR 400

Sheep Polyclonal anti-Human CCL-16 (HCC-4)
hAP-5707	Angio Proteomie	50ug	EUR 400

Mouse Monoclonal anti-human CCL-23 (MPIF-1)
hAP-0148	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-23 (MPIF-1)
hAP-0148A	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-14a (HCC-1)
hAP-5705	Angio Proteomie	50ug	EUR 400

Sheep Polyclonal anti-Human CCL-23 (MPIF-1)
hAP-5712	Angio Proteomie	50ug	EUR 400

Mouse Monoclonal anti-human CCL-26 (Eotaxin-3)
hAP-0151	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-26 (Eotaxin-3)
hAP-5715	Angio Proteomie	50ug	EUR 400

Rat Monoclonal anti-mouse CCL-28
mAP-0179	Angio Proteomie	100ug	EUR 250

Human MDC/CCL 22 ELISA KIT
E42EH-288	EnoGene	96T/48T	Ask for price

Mouse Monoclonal anti-human CCL-2 (MCP-1)
hAP-0144	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-7 (MCP-3)
hAP-0156	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-8 (MCP-2)
hAP-0157	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-human CCL-2 (MCP-1)
hAP-5073	Angio Proteomie	50ug	EUR 400

Rat Monoclonal anti-mouse CCL-3
mAP-0180	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-4
mAP-0181	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-5
mAP-0182	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-6
mAP-0183	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-8
mAP-0184	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-9
mAP-0185	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-6
mAP-0340	Angio Proteomie	100ug	EUR 250

Human RANTES (CCL 5) Recombinant Protein
100-095	ReliaTech	20 µg	EUR 196.35
Description: RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant human RANTES is a 7.8 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Human RANTES (CCL 5) Recombinant Protein
100-095S	ReliaTech	5 µg	EUR 92.4
Description: RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant human RANTES is a 7.8 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Mouse Monoclonal anti-human CCL-19 (MIP-3 beta)
hAP-0143	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-19 (MIP-3 beta)
hAP-5710	Angio Proteomie	50ug	EUR 400

Mouse Monoclonal anti-human CCL-20 (MIP-3 alpha)
hAP-0145	Angio Proteomie	100ug	EUR 250

Sheep Polyclonal anti-Human CCL-20 (MIP-3 alpha)
hAP-5706	Angio Proteomie	50ug	EUR 400

Nori® Mouse CCL-15 (MIP-5) ELISA Kit
GR117261	Genorise Scientific	96-well	EUR 461

Rat Monoclonal anti-mouse CCL-22 (MDC)
mAP-0175	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-17 (TARC)
mAP-0170	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-25 (TECK)
mAP-0177	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-27 (CTACK)
mAP-0178	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-4 (MIP-1 beta)
hAP-1166	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-21 (6Ckine)
mAP-0174	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-3 (MIP-1 alpha)
hAP-0154	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-3 (MIP-1 alpha)
hAP-0154A	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CCL-3 (MIP-1 alpha)
hAP-0154B	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-11 (Eotaxin)
mAP-0168	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-12 (MCP-5)
mAP-0169	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-24 (Eotaxin-2
mAP-0176	Angio Proteomie	100ug	EUR 250

Rabbit anti-Escherichia coli ccl Polyclonal Antibody
MBS7164180-INQUIRE	MyBiosource	INQUIRE	Ask for price

Rat Monoclonal anti-mouse CCL-1 (TCA-3)
mAP-0167	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-2 (MCP-1)
mAP-0172	Angio Proteomie	100ug	EUR 250

Human RANTES (CCL 5, Animal Free) Recombinant Protein
100-095-AF	ReliaTech	20 µg	EUR 229.95
Description: RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant human RANTES is a 7.8 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Human RANTES (CCL 5, Animal Free) Recombinant Protein
100-095S-AF	ReliaTech	5 µg	EUR 134.4
Description: RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant human RANTES is a 7.8 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Rat Monoclonal anti-mouse CCL-19 (MIP-3 beta)
mAP-0171	Angio Proteomie	100ug	EUR 250

Rat Monoclonal anti-mouse CCL-20 (MIP-3 alpha)
mAP-0173	Angio Proteomie	100ug	EUR 250

Human C-C motif chemokine 2 (CCL-2) ELISA Kit
MBS9305815-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 6725

Human C-C motif chemokine 2 (CCL-2) ELISA Kit
MBS9305815-48StripWells	MyBiosource	48-Strip-Wells	EUR 550

Human C-C motif chemokine 2 (CCL-2) ELISA Kit
MBS9305815-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 3420

Human C-C motif chemokine 2 (CCL-2) ELISA Kit
MBS9305815-96StripWells	MyBiosource	96-Strip-Wells	EUR 765

Rat-MIP1γ CCL-9-
QY-E11948	Qayee Biotechnology	96T	EUR 420

Anti- Interleukin-15 (IL-15) Human -BIOTIN Antibody
GWB-66F8CF	GenWay Biotech	0.025 mg	Ask for price

Rat RANTES (CCL 5)
MBS691650-0005mg	MyBiosource	0.005mg	EUR 300

Rat RANTES (CCL 5)
MBS691650-002mg	MyBiosource	0.02mg	EUR 450

Rat RANTES (CCL 5)
MBS691650-5x002mg	MyBiosource	5x0.02mg	EUR 1725

Mouse RANTES (CCL 5)
MBS691610-0005mg	MyBiosource	0.005mg	EUR 300

Mouse RANTES (CCL 5)
MBS691610-002mg	MyBiosource	0.02mg	EUR 450

Mouse RANTES (CCL 5)
MBS691610-5x002mg	MyBiosource	5x0.02mg	EUR 1725

Mouse Anti Human Interleukin-15
MBS140016-05mg	MyBiosource	0.5mg	EUR 440

Mouse Anti Human Interleukin-15
MBS140016-1mg	MyBiosource	1mg	EUR 600

Mouse Anti Human Interleukin-15
MBS140016-5x1mg	MyBiosource	5x1mg	EUR 2375

RABBIT ANTI HUMAN INTERLEUKIN-15
MBS221558-01mg	MyBiosource	0.1mg	EUR 635

RABBIT ANTI HUMAN INTERLEUKIN-15
MBS221558-5x01mg	MyBiosource	5x0.1mg	EUR 2695

Rat CCL-5 ELISA Kit
ERC0240	Abclonal	96Tests	EUR 625.2

Goat CCL-5 ELISA Kit
EGTC0240	Abclonal	96Tests	EUR 625.2

Sheep CCL-5 ELISA Kit
ESC0240	Abclonal	96Tests	EUR 625.2

Mouse CCL-5 ELISA Kit
EMC0240	Abclonal	96Tests	EUR 625.2

Bovine CCL-5 ELISA Kit
EBC0240	Abclonal	96Tests	EUR 625.2

Canine CCL-5 ELISA Kit
ECC0240	Abclonal	96Tests	EUR 625.2

Rabbit CCL-5 ELISA Kit
ERTC0240	Abclonal	96Tests	EUR 625.2

Monkey CCL-5 ELISA Kit
EMKC0240	Abclonal	96Tests	EUR 625.2

Chicken CCL-5 ELISA Kit
ECKC0240	Abclonal	96Tests	EUR 625.2

Porcine CCL-5 ELISA Kit
EPC0240	Abclonal	96Tests	EUR 625.2

Anserini CCL-5 ELISA Kit
EAC0240	Abclonal	96Tests	EUR 625.2

human anti-human IL-15 mAb(1A)
E4A09D21-1A	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human IL-15 mAb(2B)
E4A09D21-2B	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human IL-15 mAb(7C)
E4A09D21-7C	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human IL-15 mAb(9E)
E4A09D21-9E	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human GDF-15 mAb (FD)
E4A09G02-hFD	EnoGene	50ug	EUR 255
Description: Available in various conjugation types.

human anti-human GDF-15 mAb (FP)
E4A09G02-hFP	EnoGene	50ug	EUR 255
Description: Available in various conjugation types.

human anti-human GDF-15 mAb (H2)
E4A09G02-hH2	EnoGene	50ug	EUR 255
Description: Available in various conjugation types.

human anti-human GDF-15 mAb(32)
E4A09G02-h32	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human GDF-15 mAb(39)
E4A09G02-h39	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human GDF-15 mAb(82)
E4A09G02-h82	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Eotaxin / CCL-11 (Mouse) - ELISA Kit
EK-033-40	PHOENIX PEPTIDE	96 wells	EUR 603.72

Guinea Pig CCL-5 ELISA Kit
EGC0240	Abclonal	96Tests	EUR 625.2

Recombinant Escherichia coli Cloacin (ccl)
MBS1213221-002mgEColi	MyBiosource	0.02mg(E-Coli)	EUR 1140

Recombinant Escherichia coli Cloacin (ccl)
MBS1213221-002mgYeast	MyBiosource	0.02mg(Yeast)	EUR 1180

Recombinant Escherichia coli Cloacin (ccl)
MBS1213221-01mgEColi	MyBiosource	0.1mg(E-Coli)	EUR 1335

Recombinant Escherichia coli Cloacin (ccl)
MBS1213221-01mgYeast	MyBiosource	0.1mg(Yeast)	EUR 1390

Recombinant Escherichia coli Cloacin (ccl)
MBS1213221-002mgBaculovirus	MyBiosource	0.02mg(Baculovirus)	EUR 1420

Mouse Anti Human Interferon stimulated gene 15
MBS140275-0005mg	MyBiosource	0.005mg	EUR 240

Mouse Anti Human Interferon stimulated gene 15
MBS140275-002mg	MyBiosource	0.02mg	EUR 310

Mouse Anti Human Interferon stimulated gene 15
MBS140275-01mg	MyBiosource	0.1mg	EUR 610

Mouse Anti Human Interferon stimulated gene 15
MBS140275-5x01mg	MyBiosource	5x0.1mg	EUR 2405

Rabbit Anti Human Interleukin-15 Polyclonal Antibody
CPBT-65643RH	Creative Diagnostics	0.1 mg	EUR 1057.2

Cefpodoxime/ Clavulanic acid CCL 10/5
SD255-5CT	EWC Diagnostics	1 unit	EUR 7.89
Description: Cefpodoxime/ Clavulanic acid CCL 10/5

Cefpodoxime/ Clavulanic acid CCL 10/5
SD255-5VL	EWC Diagnostics	1 unit	EUR 14.2
Description: Cefpodoxime/ Clavulanic acid CCL 10/5

Rat RANTES (CCL 5) Recombinant Protein
R20-012	ReliaTech	20 µg	EUR 196.35
Description: RANTES is a protein which has been shown to be a chemoattractant for peripheral blood monocytes. It appears to selectively attract T cells of the CD4+/CD45RO+ phenotype in vitro. Recombinant Rat RANTES is a 7.9 kDa protein containing 68 amino acid residues.

Rat RANTES (CCL 5) Recombinant Protein
R20-012S	ReliaTech	5 µg	EUR 92.4
Description: RANTES is a protein which has been shown to be a chemoattractant for peripheral blood monocytes. It appears to selectively attract T cells of the CD4+/CD45RO+ phenotype in vitro. Recombinant Rat RANTES is a 7.9 kDa protein containing 68 amino acid residues.

Mouse RANTES (CCL 5) Recombinant Protein
M10-038	ReliaTech	20 µg	EUR 196.35
Description: RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant murine RANTES is a 7.8 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Mouse RANTES (CCL 5) Recombinant Protein
M10-038S	ReliaTech	5 µg	EUR 92.4
Description: RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant murine RANTES is a 7.8 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

The ribotoxin-like protein Ostreatin from Pleurotus ostreatus fruiting our bodies: Affirmation of a novel ribonuclease household expressed in basidiomycetes

Fungi produce a number of toxins energetic towards crops, animal or people. Amongst them, ribotoxins are enzymes that particularly assault ribosomes irreparably compromising protein synthesis, helpful as pesticides or as anticancer brokers. Right here, a novel ribotoxin from the edible mushroom Pleurotus ostreatus has been purified and characterised.

This ribotoxin, named Ostreatin, is a particular ribonuclease releasing α-fragment when incubated with yeast or rabbit ribosomes. Ostreatin exhibits IC₅₀ of 234 pM in rabbit reticulocyte lysate, and metallic dependent endonuclease exercise. Following the completion of Ostreatin major construction, we ascertained that this toxin is homologous to Ageritin, the primary ribotoxin-like protein from the basidiomycete Agrocybe aegerita, with which it shares 38.8% amino acid sequence identification.

 Ostreatin consists of 131 amino acid residues with an experimental molecular mass of 14,263.51 Da ([M+H⁺]⁺). Homology modeling revealed that Ostreatin and Ageritin share an analogous fold by which the widespread catalytic triad is conserved.

Purified Ostreatin lacks N-terminal and C-terminal peptides, which as an alternative are current within the Ostreatin coding sequence. Such peptides are in all probability concerned in protein sorting and for this they might be eliminated. Our findings affirm the presence of ribotoxin-like proteins in basidiomycetes edible mushrooms, that we suggest as novel instrument for biotechnological functions.

Characterization of differentially expressed plasma  proteins in sufferers with acute myocardial infarction

Acute myocardial infarction (AMI) stays a number one reason for morbidity and mortality worldwide. Novel biomarkers are wanted to establish NSTEMI in AMI sufferers. The research goal was to make use of proteomics to establish novel plasma biomarkers for STEMI and NSTEMI sufferers. iTRAQ evaluation was carried out on pooled samples from Eight wholesome controls and 12 STEMI and 12 NSTEMI sufferers.

 Bioinformatics evaluation recognized 95 differentially expressed proteins that had been differentially expressed within the plasma of AMI sufferers and wholesome controls; 28 of those proteins had been present in STEMI/Con (22 upregulated and 6 downregulated), 48 in NSTEMI/Con (12 upregulated and 36 downregulated), and 44 in NSTEMI/STEMI (11 upregulated and 33 downregulated). Protein community evaluation was then carried out utilizing STRING software program.

Practical evaluation revealed that the recognized plasma proteins had been primarily concerned with carbon metabolism, toll-like receptor signaling pathway, and hypertrophic cardiomyopathy. 9 of the proteins (SSA1, MDH1, FCN2, GPI, S100A8, LBP, vinculin, VDBP, and RBP4) that modified ranges throughout AMI development had been additional validated by ELISA. The constructed plasma proteome may mirror the AMI pathogenesis molecular mechanisms and supply a technique for the early identification of NSTEMI in AMI sufferers. SIGNIFICANCE:

The goal of this research was to make use of proteomics to establish novel predictive plasma biomarkers for sufferers with acute myocardial infarction (AMI), which might enable for both identification of people liable to an infarction, and early identification of NSTEMI in sufferers with AMI. Utilizing an strategy that mixed iTRAQ with LC-MS/MS, we discovered 95 proteins that confirmed vital variations in expression ranges among the many AMI sufferers and wholesome controls.

The proteins SSA1, MDH1, FCN2, GPI, S100A8, LBP, vinculin, VDBP, and RBP4 had been discovered to play essential roles within the pathogenesis of AMI. Utilizing bioinformatics evaluation, we discovered that dysregulation of carbon metabolism, toll-like receptor signaling pathway, and hypertrophic cardiomyopathy would be the main driving forces for cardiac harm throughout myocardial infarction.

Nonetheless, additional investigations are wanted to confirm the mechanisms concerned within the improvement of AMI particularly NSTEMI. Taken collectively, our findings lay the muse for understanding the molecular mechanisms underlying the pathogenic processes of AMI, and recommend potential functions for particular biomarkers in early prognosis and willpower of prognosis.

2 × Taq Master Mix
P111-02	Vazyme	15 ml	EUR 98.42

2 × Taq Master Mix
P111-03	Vazyme	50 ml	EUR 290

Taq 2X Master Mix
MBS308023-1000Reactions	MyBiosource	1000Reactions	EUR 340

Taq 2X Master Mix
MBS308023-100Reactions	MyBiosource	100Reactions	EUR 165

Taq 2X Master Mix
MBS308023-250Reactions	MyBiosource	250Reactions	EUR 185

Taq 2X Master Mix
MBS308023-500Reactions	MyBiosource	500Reactions	EUR 240

2 × Taq Master Mix
P111-01-51ml	Vazyme	5 × 1 ml	EUR 38.22

2 × Taq Master Mix
P111-03-501ml	Vazyme	50 × 1 ml	EUR 300.3

2X Taq Master Mix, 100app
PLMM01	Vivantis	each	Ask for price

2 × Rapid Taq Master Mix
P222-01	Vazyme	5 ml (5×1ml)	EUR 53.5

2 × Rapid Taq Master Mix
P222-02	Vazyme	15 ml (15×1ml)	EUR 144.5

2 × Rapid Taq Master Mix
P222-03	Vazyme	50 ml (50 x 1 ml)	EUR 430

2 × Rapid Taq Master Mix
P222-04	Vazyme	50 ml (10×5ml)	EUR 430

2 × Rapid Taq Master Mix
P222-01-51ml	Vazyme	5 × 1 ml	EUR 55.4

2 × Rapid Taq Master Mix
P222-02-151ml	Vazyme	15 × 1 ml	EUR 149.63

2 × Rapid Taq Master Mix
P222-03-501ml	Vazyme	50 × 1 ml	EUR 445.27

2 × Rapid Taq Master Mix
P222-04-105ml	Vazyme	10 × 5 ml	EUR 445.27

2x Taq PCR Master Mix
A4145-1ML40Rxns	Biomatik Corporation	1ML (40Rxns)	EUR 77
Description: Biotechnology

Taq PCR Master Mix (2x)
E2520-01	EURx	100reactions	EUR 38.15
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

Taq PCR Master Mix (2x)
E2520-02	EURx	200reactions	EUR 71.94
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

Taq PCR Master Mix (2x)
E2520-03	EURx	500reactions	EUR 155.87
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

Taq 2X PCR Master Mix
RK20602	Abclonal	500RXN	EUR 12.54

Taq (exo-) 2X Master Mix
RK20612	Abclonal	100RXN	EUR 49.05

Taq Mix (2x) (PCR Master Mix (2x))
50900	Sisco Laboratories	1 ml	EUR 21.38
Description: Part E

Taq Mix (2x) (PCR Master Mix (2x))
50900-1	Sisco Laboratories	5 x1 ml	EUR 64.14
Description: Part E

Taq Mix (2x) (PCR Master Mix (2x))
50900-2	Sisco Laboratories	5 x2.5 ml	EUR 128.29
Description: Part E

2 × Taq Plus Master Mix
P211-01	Vazyme	5 ml	EUR 58.81

2 × Taq Plus Master Mix
P211-02	Vazyme	15 ml	EUR 162.24

2 × Taq Plus Master Mix
P211-03	Vazyme	50 ml	EUR 507

2 × Taq Plus Master Mix
P211-01-51ml	Vazyme	5 × 1 ml	EUR 60.9

2 × Taq Plus Master Mix
P211-03-501ml	Vazyme	50 × 1 ml	EUR 525

2X ViRed Taq Master Mix, 100app
CLMM01	Vivantis	each	Ask for price

Taq 2X MeanGreen Master Mix
MBS308024-1000Reactions	MyBiosource	1000Reactions	EUR 370

Taq 2X MeanGreen Master Mix
MBS308024-100Reactions	MyBiosource	100Reactions	EUR 195

Taq 2X MeanGreen Master Mix
MBS308024-250Reactions	MyBiosource	250Reactions	EUR 215

Taq 2X MeanGreen Master Mix
MBS308024-500Reactions	MyBiosource	500Reactions	EUR 270

2 × Taq Master Mix (Dye Plus)
P112-01	Vazyme	5 ml	EUR 36.91

2 × Taq Master Mix (Dye Plus)
P112-02	Vazyme	15 ml	EUR 98.42

2 × Taq Master Mix (Dye Plus)
P112-03	Vazyme	50 ml	EUR 290

2 × Taq Master Mix (Dye Plus)
P112-01-51ml	Vazyme	5 × 1 ml	EUR 38.22

2 × Taq Master Mix (Dye Plus)
P112-02-151ml	Vazyme	15 × 1 ml	EUR 101.92

2 × Taq Master Mix (Dye Plus)
P112-03-501ml	Vazyme	50 × 1 ml	EUR 300.3

2 × EpiArt HS Taq Master Mix
EM201-01	Vazyme	1ml	EUR 16

2 × EpiArt HS Taq Master Mix
EM201-02	Vazyme	5 ml (5×1ml)	EUR 70

4 × EpiArt HS Taq Master Mix
EM201-03	Vazyme	15 ml (15×1ml)	EUR 192

Apex 2X Taq Master Mix, Clear
42-133	Genesee Scientific	100 Reactions/Unit	EUR 54.78
Description: 1.5mM MgCl2 (Final Conc.)

Apex 2X Taq Master Mix, Clear
42-134	Genesee Scientific	500 Reactions/Unit	EUR 220.95
Description: 1.5mM MgCl2 (Final Conc.)

Apex 2X Taq Master Mix, Clear
42-134B	Genesee Scientific	1000 Reactions/Unit	EUR 433.29
Description: 1.5mM MgCl2 (Final Conc.)

Roseate PCR Master Mix (2x) (Taq Mix (2x))
46503	Sisco Laboratories	1 ml	EUR 23.09
Description: Part E

Roseate PCR Master Mix (2x) (Taq Mix (2x))
46503-1	Sisco Laboratories	5 x1 ml	EUR 70.13
Description: Part E

Roseate PCR Master Mix (2x) (Taq Mix (2x))
46503-2	Sisco Laboratories	5 x2.5 ml	EUR 145.39
Description: Part E

Apex Taq RED Master Mix, 2X
42-137	Genesee Scientific	100 Reactions/Unit	EUR 58.5
Description: 1.5mM MgCl2 (Final Conc.)

Apex Taq RED Master Mix, 2X
42-138	Genesee Scientific	500 Reactions/Unit	EUR 221.56
Description: 1.5mM MgCl2 (Final Conc.)

Apex Taq RED Master Mix, 2X
42-138B	Genesee Scientific	1000 Reactions/Unit	EUR 437.28
Description: 1.5mM MgCl2 (Final Conc.)

Color Taq PCR Master Mix (2x)
E2525-01	EURx	100reactions	EUR 38.15
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized and colored reaction buffer, MgCl2 and dNTPs

Color Taq PCR Master Mix (2x)
E2525-02	EURx	200reactions	EUR 71.94
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized and colored reaction buffer, MgCl2 and dNTPs

Color Taq PCR Master Mix (2x)
E2525-03	EURx	500reactions	EUR 155.87
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized and colored reaction buffer, MgCl2 and dNTPs

ViPrimePLUS Taq qPCR Master Mix, 150rxns
QLMM11	Vivantis	each	Ask for price

HotStart Taq 2X PCR Master Mix
RK20603	Abclonal	500RXN	EUR 20.17

HotStart Taq (exo-) 2X Master Mix
RK20613	Abclonal	100RXN	EUR 100.83

Perpetual Taq PCR Master Mix (2x)
E2740-01	EURx	100reactions	EUR 90.47
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies,optimized reaction buffer, MgCl2 and dNTPs

Perpetual Taq PCR Master Mix (2x)
E2740-02	EURx	200reactions	EUR 174.4
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies,optimized reaction buffer, MgCl2 and dNTPs

Perpetual Taq PCR Master Mix (2x)
E2740-03	EURx	500reactions	EUR 347.71
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies,optimized reaction buffer, MgCl2 and dNTPs

LongerAmp Taq 2X PCR Master Mix
RK20682	Abclonal	100RXN	EUR 198.93

Taq PCR Master Mix (2X, Red Dye)
BS9297	Bio Basic	1ml	EUR 101.76

Taq PCR Master Mix (2X, Red Dye)
BS9298	Bio Basic	5ml	EUR 227.04

Taq PCR Master Mix (2X, Blue Dye)
BS9295	Bio Basic	1ml	EUR 101.76

Taq PCR Master Mix (2X, Blue Dye)
BS9296	Bio Basic	5ml	EUR 227.04

2X Taq PCR Master Mix (with dye)
K1034-1	ApexBio	1 ml	EUR 16

2X Taq PCR Master Mix (with dye)
K1034-100	ApexBio	100×1 ml	EUR 512

2X Taq PCR Master Mix (with dye)
K1034-20	ApexBio	20×1 ml	EUR 160

2X Taq PCR Master Mix (with dye)
K1034-5	ApexBio	5×1 ml	EUR 48

2X Taq PCR Master Mix (with dye)
K1034-50	ApexBio	50×1 ml	EUR 320

Taq 2X PCR Master Mix with Dye
RK20604	Abclonal	500RXN	EUR 89.93

Taq (exo-) 2X Master Mix with Dye
RK20614	Abclonal	500RXN	EUR 12.54

2 × Taq Plus Master Mix (Dye Plus)
P212-01	Vazyme	5 ml	EUR 159.6

2 × Taq Plus Master Mix (Dye Plus)
P212-02	Vazyme	15 ml	EUR 231.6

2 × Taq Plus Master Mix (Dye Plus)
P212-03	Vazyme	50 ml	EUR 466.8

3G Taq Master Mix for PAGE (Red Dye)
P115-01	Vazyme	5 ml	EUR 38.67

3G Taq Master Mix for PAGE (Red Dye)
P115-02	Vazyme	10 × 5 ml	EUR 290

3G Taq Master Mix for PAGE (Red Dye)
P115-02-105ml	Vazyme	10 × 5 ml	EUR 300.3

2 × EpiArt HS Taq Master Mix (Dye Plus)
EM202-01	Vazyme	1ml	EUR 16

2 × EpiArt HS Taq Master Mix (Dye Plus)
EM202-02	Vazyme	5 ml (5×1ml)	EUR 70

2 × EpiArt HS Taq Master Mix (Dye Plus)
EM202-03	Vazyme	15 ml (15×1ml)	EUR 192

Color Perpetual Taq PCR Master Mix (2x)
E2745-01	EURx	100reactions	EUR 90.47
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies, optimizedand colored reaction buffer, MgCl2 and dNTPs

Color Perpetual Taq PCR Master Mix (2x)
E2745-02	EURx	200reactions	EUR 174.4
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies, optimizedand colored reaction buffer, MgCl2 and dNTPs

Color Perpetual Taq PCR Master Mix (2x)
E2745-03	EURx	500reactions	EUR 347.71
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies, optimizedand colored reaction buffer, MgCl2 and dNTPs

Taq Pro Universal SYBR qPCR Master Mix
Q712-02	Vazyme	500 rxns (20 μl/rxn)	EUR 161

Taq Pro Universal SYBR qPCR Master Mix
Q712-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 725

Taq Pro Universal SYBR qPCR Master Mix
Q712-00-100rxns	Vazyme	100 rxns	EUR 1.09

Taq Pro Universal SYBR qPCR Master Mix
Q712-02-500rxns	Vazyme	500 rxns	EUR 166.72

Taq Pro Universal SYBR qPCR Master Mix
Q712-03-2500rxns	Vazyme	2,500 rxns	EUR 750.74

Accuris Taq Master Mix, 2X conc., 1000 reactions
PR1001-1000	Benchmark Scientific	1 PC	EUR 375.85

Accuris Taq Master Mix, 2X conc., 200 reactions
PR1001-200	Benchmark Scientific	1 PC	EUR 89.71

2 × Taq Plus Master Mix Ⅱ (Dye Plus)
P213-01	Vazyme	5 ml	EUR 58.81

2 × Taq Plus Master Mix Ⅱ (Dye Plus)
P213-02	Vazyme	15 ml	EUR 162.24

2 × Taq Plus Master Mix Ⅱ (Dye Plus)
P213-03	Vazyme	50 ml	EUR 507

2 × Taq Plus Master Mix II (Dye Plus)
P213-01-51ml	Vazyme	5 × 1 ml	EUR 60.9

2 × Taq Plus Master Mix II (Dye Plus)
P213-02-151ml	Vazyme	15 × 1 ml	EUR 168

2 × Taq Plus Master Mix II (Dye Plus)
P213-03-501ml	Vazyme	50 × 1 ml	EUR 525

2×Fast Taq PCR Master Mix (with dye)
K1116-1	ApexBio	1ml	EUR 40
Description: Molecular Biology|Reverse Transcription & PCR

2×Fast Taq PCR Master Mix (with dye)
K1116-100	ApexBio	100x1ml	EUR 736
Description: Molecular Biology|Reverse Transcription & PCR

2×Fast Taq PCR Master Mix (with dye)
K1116-20	ApexBio	20x1ml	EUR 240
Description: Molecular Biology|Reverse Transcription & PCR

2×Fast Taq PCR Master Mix (with dye)
K1116-5	ApexBio	5x1ml	EUR 84
Description: Molecular Biology|Reverse Transcription & PCR

2×Fast Taq PCR Master Mix (with dye)
K1116-50	ApexBio	50x1ml	EUR 464
Description: Molecular Biology|Reverse Transcription & PCR

Apex Hot Start Taq BLUE Master Mix, 2X
42-143	Genesee Scientific	100 Reactions/Unit	EUR 65.77
Description: 1.5mM MgCl2 (Final)

Apex Hot Start Taq BLUE Master Mix, 2X
42-144	Genesee Scientific	500 Reactions/Unit	EUR 288.71
Description: 1.5mM MgCl2 (Final)

Apex Hot Start Taq BLUE Master Mix, 2X
42-145	Genesee Scientific	1000 Reactions/Unit	EUR 470.2
Description: 1.5mM MgCl2 (Final)

Apex Hot Start Taq BLUE Master Mix, 2X
42-146	Genesee Scientific	2500 Reactions/Unit	EUR 1216.89
Description: 1.5mM MgCl2 (Final), Buffer I

Apex Hot Start Taq BLUE Master Mix, 2X
42-147	Genesee Scientific	100 Reactions/Unit	EUR 61.77
Description: 1.5mM MgCl2 (Final), Buffer II

Apex Hot Start Taq BLUE Master Mix, 2X
42-148	Genesee Scientific	500 Reactions/Unit	EUR 253.97
Description: 1.5mM MgCl2 (Final), Buffer II

Apex Hot Start Taq BLUE Master Mix, 2X
42-149	Genesee Scientific	1000 Reactions/Unit	EUR 473.03
Description: 1.5mM MgCl2 (Final), Buffer II

Apex Hot Start Taq BLUE Master Mix, 2X
42-150	Genesee Scientific	2500 Reactions/Unit	EUR 1072.65
Description: 1.5mM MgCl2 (Final), Buffer II

ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns
QLMM12	Vivantis	each	Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns
QLMM17	Vivantis	each	Ask for price

HotStart Taq 2X PCR Master Mix with Dye
RK20605	Abclonal	500RXN	EUR 21.8

2X OneStep Taq ReverseTrans PCR Master Mix, 100apps
RTMM01	Vivantis	each	Ask for price

LongerAmp Taq 2X PCR Master Mix with Dye
RK20684	Abclonal	500RXN	EUR 51.78

ViPrimePLUS OneStep Taq RT-qPCR Master Mix, 150rxns
QLMM13	Vivantis	each	Ask for price

Accuris Taq Master Mix, 2X conc., sample, 5 reactions
PR1001-S	Benchmark Scientific	1 PC	EUR 7.31

ViPrimePLUS Taq qPCR Master Mix with ROX, 150rxns
QLMM11-R	Vivantis	each	Ask for price

2X ViRed OneStep Taq ReverseTrans PCR Master Mix, 100apps
RTMM02	Vivantis	each	Ask for price